Comparison of the optimal and suboptimal quantity of mitotype libraries using next-generation sequencing.

Optimizing analysis parameters and sample input is crucial in forensic genetics methods to generate reliable results, and even more so when working with muti-copy mitochondrial DNA (mtDNA) and low-quality samples. This study compared mitotypes based on next-generation sequencing (NGS) results derived from the same samples at two different sequencing library concentrations-30 pM and 0.3 pM. Thirty femur samples from the Second World War were used as a model for poorly preserved DNA. Quantitative PCR (qPCR) method targeting 113 bp long fragment was employed to assess the quantity of mitogenomes. HID Ion Chef™ Instrument with Precision ID mtDNA Control Region Panel was used for library preparation and templating. Sequencing was performed with Ion GeneStudio™ S5 System. Reference haplotypes were determined from sequencing samples at 30 pM library input. Haplotypes were compared between optimal (30 pM) and suboptimal (0.3 pM) library inputs. Often the difference in haplotypes was length heteroplasmy, which in line with other studies shows that this type of variant is not reliable for interpretation in forensics. Excluding length variants at positions 573, 309, and 16,193, 56.7% of the samples matched, and in two samples, no sequence was obtained at suboptimal library input. The rest of the samples differed between optimal and suboptimal library input. To conclude, genotyping and analyzing low-quantity libraries derived from low-quality aged skeletonized human remains therefore must be done with caution in forensic genetics casework.

Sir Benjamin William Rycroft OBE (1902-1967): British ophthalmologist and pioneer in corneal surgery.

An unsung hero of British ophthalmology is the largely forgotten Sir Benjamin Rycroft (1902-1967). This paper will discuss and analyse the undervalued career of this great man. Upon graduating from medical school, Rycroft became a General Practitioner. Rycroft then decided to train to become an ophthalmologist. Rycroft began his ophthalmology career in 1930s London focusing on the new ground-breaking surgery of keratoplasty (corneal grafting) before serving with distinction in the medical corps during the Second World War. He is chiefly remembered for his work after the war at the Queen Victoria Hospital in East Grinstead, Sussex, where he worked with renowned plastics surgeon Archibald McIndoe. During his time, there Rycroft became globally recognised for his skill in keratoplasty and started a campaign which radically changed the legal framework behind organ donation in the UK. Despite few knowing of him today, Rycroft is undoubtedly one of the most influential British ophthalmologists of the past century. He was for decades seen as one of the world's leading practitioners of keratoplasty and established a unit which restored sight to wounded veterans. His greatest achievement lies in his organ donation reform, which started the process of allowing organ donation to be carried out on a nationwide scale for the first time.

Caring Under Fire Across Three Continents: The Hadfield-Spears Ambulance, 1941-1945.

During the Second World War, the Hadfield Spears ambulance took care of around 22,000 wounded and/or sick patients across three continents. This article analyses how military attacks and instances of violence impacted on the psychological, emotional and physical health of those attending the wounded within this mobile unit. While historiography of allied medicine develops apace, analysis of the Free French health service remains rare. Yet the history of the Hadfield Spears ambulance provides a fascinating window into the neglected issue of attacks on healthcare in wartime, as well as a fresh scope for combining macro and micro perspectives. The deployment of both approaches suggests potent ways to connect intimate responses to attacks to broader histories of allied frictions and cooperation. Crucially, it offers rich insights into the development of a transnational 'ethos of stoicism', which helped to sustain the hospital's community, in a fraught allied diplomatic context.

Measure quantity of mitochondrial DNA in aged bones or calculate it from nuclear DNA quantitative PCR results?

Mitochondrial DNA (mtDNA) is of great value in forensics to procure information about a person when a next of kin, personal belongings, or other sources of nuclear DNA (nDNA) are unavailable, or nDNA is lacking in quality and quantity. The quality and reliability of the results depend greatly on ensuring optimal conditions for the given method, for instance, the optimal input of the copy number (CN) in next-generation sequencing (NGS) methods. The unavailability of commercial quantitative PCR (qPCR) methods to determine mtDNA CN creates the necessity to rely on recommendations to infer mtDNA CN from nDNA yield. Because nDNA yield varies between individuals, tissues, parts of the same tissue, and because mtDNA CN varies between tissues, such assumptions must be examined for a specific context, rather than be generalized. This study compares mtDNA CN calculated from nDNA yield and qPCR measured mtDNA CN. Seventy-five femurs from the Second World War victims were used as samples; they were cut below the greater trochanter, surface contaminants were removed by mechanical and chemical cleaning, samples were fully demineralized, and DNA was isolated. PowerQuant® Kit (Promega) was used to analyze DNA yield. An in-house method was used to determine mtDNA CN. Comparison of mtDNA CN from nDNA derived calculations and measured mtDNA CN highlighted vast differences. The results emphasize the need to perform qPCR to assess mtDNA CN before NGS analyses of aged bones' mitogenomes rather than estimating mtDNA CN from nDNA yield to ensure the quality and reliability of the results of NGS analysis.

A Multisample Approach in Forensic Phenotyping of Chronological Old Skeletal Remains Using Massive Parallel Sequencing (MPS) Technology.

It is very important to generate phenotypic results that are reliable when processing chronological old skeletal remains for cases involving the identification of missing persons. To improve the success of pigmentation prediction in Second World War victims, three bones from each of the eight skeletons analyzed were included in the study, which makes it possible to generate a consensus profile. The PowerQuant System was used for quantification, the ESI 17 Fast System was used for STR typing, and a customized version of the HIrisPlex panel was used for PCR-MPS. The HID Ion Chef Instrument was used for library preparation and templating. Sequencing was performed with the Ion GeneStudio S5 System. Identical full profiles and identical hair and eye color predictions were achieved from three bones analyzed per skeleton. Blue eye color was predicted in five skeletons and brown in three skeletons. Blond hair color was predicted in one skeleton, blond to dark blond in three skeletons, brown to dark brown in two skeletons, and dark brown to black in two skeletons. The reproducibility and reliability of the results proved the multisample analysis method to be beneficial for phenotyping chronological old skeletons because differences in DNA yields in different bone types provide a greater possibility of obtaining a better-quality consensus profile.

A fast and highly efficient automated DNA extraction method from small quantities of bone powder from aged bone samples.

An efficient extraction method is important for obtaining high-quality DNA from degraded aged bone samples. An automated full-demineralization method using the EDTA and DNA Investigator Kit (Qiagen) combined with Qiagen's biorobots was optimized in our laboratory in the past to extract the DNA from 500 mg of aged bone samples. The purpose of this research was to further improve the method with the aim of reducing the required sample material, shortening the extraction time, and achieving higher throughput. To process extremely small samples, the amount of bone powder was reduced to 75 mg, EDTA was replaced with reagents from the Bone DNA Extraction Kit (Promega), and decalcification was shortened from overnight to 2.5 h. Instead of 50 ml tubes, 2 ml tubes were used, which allows higher throughput. The DNA Investigator Kit (Qiagen) and EZ1 Advanced XL biorobot (Qiagen) was used for DNA purification. A comparison between both extraction methods was made on 29 Second World War bones and 22 archaeological bone samples. The differences between both methods were explored by measuring nuclear DNA yield and STR typing success. After cleaning the samples, 500 mg of bone powder was processed using EDTA, and 75 mg of powder from the same bone was processed using the Bone DNA Extraction Kit (Promega). DNA content and DNA degradation were determined using PowerQuant (Promega), and the PowerPlex ESI 17 Fast System (Promega) was used for STR typing. The results showed that the full-demineralization protocol using 500 mg of bone was efficient for Second World War and archaeological samples, and the partial-demineralization protocol using 75 mg of bone powder was only efficient for the Second World War bones. The improved extraction method-for which significantly lower amounts of bone powder can be used, the extraction process is faster, and higher throughput of bone samples is possible-is applicable for genetic identification of relatively well-preserved aged bone samples in routine forensic analyses.

Dermatopathology in Strasbourg during the German Occupation (1940-1945).

INTRODUCTION: The activity of the Strasbourg Dermatology Clinic was interrupted in September 1939 by the outbreak of the Second World War and the evacuation of the hospital. After annexing Alsace to the Reich, the German authorities demanded that physicians return to work, which resumed at the Dermatology Clinic, and was now entirely Germanized, in particular the laboratory of dermatopathology. Our aim was to study activity in the histopathology laboratory between 1939 and 1945. MATERIAL AND METHODS: We studied all the histopathology reports contained in three registers written in German. We collected patient data, clinical elements and diagnoses by microscopy. There were a total of 1202 cases between September 1940 and March 1945. The records were in a good state of preservation, enabling exhaustive analysis. RESULTS: The number of cases peaked in 1941 and diminished thereafter. The average age of patients was 49 years, and the sex ratio was 0.77. Patients were referred from Alsace or other Reich territories; referrals from other regions of France or other countries had ceased. There were 655 cases in dermatopathology, with a predominance of tumor lesions, followed by infections and inflammatory dermatoses. We noted 547 cases of non-cutaneous diseases, mainly in gynecology, urology, and in ear, nose, throat and digestive surgery; their numbers peaked in 1940-41, then tapered off progressively. DISCUSSION: Thedisruptions associated with thewar were manifested by the use of German language and the cessation of scientific publications. The lack of general pathologists in the hospital resulted in numerous cases in general pathology. Skin biopsies were mainly diagnostic and focused on skin cancers, whereas inflammatory and infectious diseases predominated before the war. No traces of data related to unethical human experimentation were identified in these archives, in contrast to other institutes in Strasbourg that were truly Nazified. CONCLUSION: These data from the Strasbourg Dermatology Clinic contain valuable information for the history of medicine and provide an insight into the functioning of a laboratory under the Occupation.

Surgery on the battlefield: Mobile surgical units in the Second World War and the memoirs they produced.

In the Second World War, there was a flowering of the battlefield surgery pioneered in the Spanish Civil War. There were small, mobile surgical units in all the theatres of the War, working close behind the fighting and deployed flexibly according to the nature of the conflict. With equipment transported by truck, jeep or mule, they operated in tents, bunkers and requisitioned buildings and carried out abdominal, thoracic, head and neck, and limb surgery. Their role was to save life and to ensure that wounded soldiers were stable for casualty evacuation back down the line to a base hospital. There is a handful of memoirs by British doctors who worked in these units and they make enthralling reading. Casualty evacuation by air replaced the use of mobile surgical units in later wars, throwing into doubt their future relevance in the management of battle wounds. But recent re-evaluations by military planners suggest that their mobility still gives them a place, so the wartime memoirs may have more value than simply as war stories.

Las diversas caras de la enfermería durante el Nacionalsocialismo (1933-1945): una revisión narrativa / As várias faces da enfermagem durante o nacional-socialismo (1933-1945): uma revisão narrativa / he diverse faces of nursing during National Socialism (1933-1945): a narrative review

In a certain period in the history of nursing, the care and attention characteristic of theprofession coexisted with the ignorance and cruelty of a society corrupted by tragedy and hatredtowards the human being. Objective: to make known the role of the supporters of the Nazi party asnurses of the Allied side and of the resistance during National Socialism in Europe and expose thedifferences between the care they provided. Methodology: narrative review developed through thebibliographic search of books and articles in various databases, selected based on inclusion andexclusion criteria. Results: the nursing care of the time was strongly differentiated between twosides, where empathy and affection were replaced by abuse and barbarism. In addition, a type ofnursing has been discovered which was forced to help the National Socialist regime against its will.Conclusion: the actions of those nurses who worked during the time of Nazism have been reflected,for the current nursing can know what happened in the past to learn and improve for the future.(AU)

En un determinado período de la historia de la enfermería, los cuidados característicosde la profesión convivieron junto la ignorancia y la crueldad de una sociedad corrompida por latragedia, el miedo y el odio. Objetivo: dar a conocer tanto el papel de las enfermeras simpatizantesdel partido nazi como el de las enfermeras del bando aliado y de la resistencia durante el Nacionalsocialismo en Europa y exponer las diferencias entre los cuidados que prestaron. Metodología: revisión narrativa desarrollada a través de la búsqueda bibliográfica de libros y de artículos en diversas bases de datos, seleccionados a partir de unos criterios de inclusión y exclusión. Resultados: laatención enfermera de la época estuvo fuertemente diferenciada entre los dos bandos, dónde la empatía y el afecto se substituyeron por el maltrato y la barbarie. Además, se ha descubierto un tipode enfermería la cual se vio obligada a ayudar al régimen nacionalsocialista en contra de su voluntad. Conclusión: se han plasmado las actuaciones de aquellas enfermeras que trabajaron durante laépoca del nazismo, para que la enfermería actual pueda conocer lo que ocurrió en el pasado y mejorar de cara al futuro.(AU)

Num determinado período da história da enfermagem, o cuidado e a atenção característicada profissão coexistiam com a ignorância e a crueldade de uma sociedade corrompida pela tragédiae pelo ódio ao ser humano. Objectivo: dar a conhecer o papel dos apoiantes do partido nazi comoenfermeiros do lado aliado e da resistência durante o nacional-socialismo na Europa o expor as diferenças entre os cuidados que prestaram. Metodologia: revisão narrativa desenvolvida através dabusca bibliográfica de artigos em várias bases de dados, selecionados com base em critérios de inclusão e exclusão. Resultados: o cuidado de enfermagem do tempo foi fortemente diferenciado entreos dois lados, onde a empatia e o afeto foram substituídos por abuso e barbárie. Além disso, foidescoberto um tipo de enfermagem que foi forçada a ajudar o regime nacional-socialista contra suavontade. Conclusão: as ações das enfermeiras que trabalharam na época do nazismo foram reflectidas, para que a enfermagem atual possa saber o que aconteceu no passado para aprender e melhorarpara o futuro.(AU)

Hematologist Bracha Ramot (1927-2006): Between the bedside and the bench.

The Hebrew University of Jerusalem opened the first medical school in Israel in May 1949. One of the select 45 students of its first class was Bracha (Chweidan) Ramot. After completing her medical studies with distinction, she went on to specialize in internal medicine and hematology and soon became a central figure in the development of hematology in Israel. In 1958, Ramot established the Hematological Institute at Tel-Hashomer hospital and served as its director until 1991. She devoted much of her time and effort to researching environmental and genetic factors that influence hematological conditions: deficiencies in coagulation factors, glucose metabolism disorders, and especially leukemias and lymphomas, including the type known as Hodgkin's disease. In 2001, Ramot, "The Doyenne of Israeli Hematology" as she was called in publications of the Albert Einstein Institute, was awarded the Israel Prize in Medical Sciences, the country's most prestigious prize. Her biography personifies the ability to overcome obstacles and challenges in one's personal life while concurrently becoming an exceedingly successful physician and researcher of extraordinary achievement.

Comparison of DNA preservation between ribs and vertebrae.

The choice of skeletal element types and their intra-bone parts is important because of differences in DNA preservation, and this must be considered when sampling bones for DNA testing. When incomplete skeletons are found, ribs and vertebrae have been shown to be the most suitable for genetic identification of bones from the torso. This study compares the preservation of DNA between 12th thoracic vertebrae and first ribs to determine which bone type is more suitable for genetic typing. The study analyzed 35 12th thoracic vertebrae and 29 first ribs from one mass grave from the Second World War with commingled skeletal remains excavated. Bone DNA preservation was estimated by measuring nuclear DNA concentration and its degradation and through short tandem repeat (STR) typing success. Previous studies performed on aged skeletal remains have shown that the DNA content of the first ribs and 12th thoracic vertebrae has high intra-bone variability, and this was considered when sampling the bones. After full demineralization extraction, the PowerQuant System (Promega) was used to measure the quantity and quality of DNA, and the GlobalFiler kit (Applied Biosystems) was used for STR typing. The results showed that DNA yield and degradation and STR typing success exhibited no statistically significant difference between first ribs and 12th thoracic vertebrae, and there was no intra-individual difference when comparing only paired bones from the same individuals. Consequently, with intra-bone DNA variability considered, the first ribs or the 12th thoracic vertebrae can be selected when sampling to genetically identify the skeletal remains of highly degraded torsos. HIGHLIGHTS: The first ribs and thoracic vertebrae are the most suitable bones for sampling from the torso. The proximal part of first rib and posterior vertebral column of the 12th thoracic vertebrae yielded the most DNA. The first ribs were compared with the 12th thoracic vertebrae, and the sampling process considered intra-bone DNA variability. The quality and quantity of nuclear DNA and success of STR typing were measured. The first ribs yielded the same DNA yields as well as STR typing success as the 12th thoracic vertebrae. When only the torso is present, it is not of high importance whether the first ribs or the 12th thoracic vertebrae are collected.

Uma enfermeira da Força Expedicionária Brasileira na Segunda Guerra Mundial: Fundo Virgínia Portocarrero da Casa de Oswaldo Cruz/Fiocruz / A nurse from the Brazilian Expeditionary Force in the Second World War: the Virgínia Portocarrero archive at Casa de Oswaldo Cruz/Fiocruz

Resumo Objetivou-se apontar as potencialidades do Fundo Virgínia Portocarrero, da Casa de Oswaldo Cruz/Fiocruz. O acervo foi doado em vida por sua titular, enfermeira brasileira veterana da Segunda Guerra Mundial. Dessa documentação constam registros de sua formação profissional e acadêmica, e é percebida sua determinação em preservar as reminiscências do front de batalha e do período pós-guerra, numa luta simbólica pelo registro de uma história essencialmente feminina em um cenário emblematicamente masculino. Integra o acervo ampla variedade de tipologias de fontes históricas, com destaque para um diário de sua participação no conflito, demonstrando aspectos cotidianos e peculiares de sua atuação profissional na saúde naquele momento dramático da história da humanidade.

Abstract This study investigates the potential of the Virgínia Portocarrero archive, at Casa de Oswaldo Cruz/Fiocruz. It was donated in life by Portocarrero, a Brazilian nurse and Second World War veteran. The documents include records from her education and training and show evidence of a determination to preserve her memories from the frontline and after the war, in a symbolic battle to record an essentially female story in a characteristically male setting. The archive contains a broad variety of types of historical sources, notably a diary on her participation in the conflict, demonstrating everyday and unusual aspects of her work as a health professional at that dramatic time in human history.

El servicio de transfusión del ejército republicano durante la Guerra Civil Española y su influencia en Gran Bretaña en la Segunda Guerra Mundial / The blood transfusion service of the republican army during the Spanish Civil War and its influence in Great Britain in World War II

Al final de la Primera Guerra Mundial, el valor de la transfusión sanguínea era ampliamente reconocido, pero no fue hasta la Guerra Civil Española (1936-1939) cuando se crearon grandes organizaciones civiles de donantes de sangre para proporcionar sangre conservada (citratada) para la transfusión en pacientes civiles y militares en ambos bandos. Se desarrollaron técnicas de transfusión indirecta para administrar esta sangre, con instrumental de fácil manejo, capaz de ser llevado a cabo en cualquier lugar y por cualquiera con experiencia en la administración de inyecciones endovenosas. También se establecieron sistemas eficaces de transporte y distribución, a fin de abastecer adecuadamente las instalaciones sanitarias que prestaban servicio en los frentes de batalla en movimiento. Este trabajo pionero permitió la creación, por primera vez en la historia, de servicios militares de transfusión de sangre. Si bien este fue el caso tanto de las fuerzas insurgentes (nacionalistas) como del Gobierno republicano español, es este último el que constituye el foco de estudio aquí. Esto es posible gracias a las publicaciones del hematólogo catalán Frederic Duran Jordà, creador y director del Servicio Republicano de Transfusión de Sangre, que detallan las técnicas y procedimientos desarrollados durante la guerra para la recogida, análisis, distribución y transfusión de sangre. Esta información se amplía, especialmente con respecto a la aplicación práctica de la transfusión en primera línea, gracias a diferentes publicaciones del Dr. Reginald Saxton, voluntario británico integrado en la Sanidad Militar de la Republica. Es nuestra intención demostrar que las autoridades británicas se beneficiaron de la experiencia española en vísperas del estallido de la Segunda Guerra Mundial en 1939. Les ayudó a desarrollar planes para los servicios de transfusión que resultarían cruciales en el tratamiento de bajas civiles y militares durante la contienda. Frederic Duran Jordà y Reginald Saxton fueron asesores clave, junto con otros que regresaban de España, incluyendo a la reconocida hematóloga británica, Janet Vaughan. Además, los principios desarrollados en España (1936-39) para la práctica militar de transfusiones de sangre siguen respaldando las medidas actuales de reanimación de heridos en el campo de batalla.

By the end of the First World War, the value of blood transfusion was widely recognized, but it was not until the Spanish Civil War (1936-1939) that large civilian blood donor organizations were created to provide preserved (citrated) blood for transfusion, for both civilian and military patients. Indirect transfusion techniques were developed to administer this blood, with easy-to-use instruments, capable of being carried out anywhere and by any medical personnel with experience in the administration of intravenous injections. Efficient transportation and distribution systems were also established in order to adequately supply the sanitary facilities that served the moving battle fronts. This pioneering work enabled the creation, for the first time in history, of military blood transfusion services. While this was the case for both the insurgent (Nationalist) forces and those of the Spanish Republican Government, it is the latter that forms the focus of this particular study. This is made possible by the publications of Catalan hematologist Frederic Duran Jordà, creator and director of the Republican Blood Transfusion Service, who details the techniques and procedures developed during the war for the collection, testing, distribution and transfusion of blood. Further insight - especially with regard to the practical application of front-line transfusion - can be gleaned from the various writings of Dr Reginald Saxton, British volunteer with the Republican Sanidad Militar. It is the intention, here, to demonstrate that the British authorities benefitted greatly from the Spanish experience in the development of plans for transfusion services that would prove crucial in treating civilian and military casualties during World War II (1939-1945). Frederic Duran Jordà, and Reginald Saxton were key advisers, together with others returning from Spain, including renowned British hematologist, Janet Vaughan. Moreover, the principles developed in Spain (1936-39) for delivery of military blood transfusion practice still support current measures in battlefield casualty resuscitation.

HISTORY OF BIOCHEMISTRY IN LVIV.

OBJECTIVE: The aim: The purpose of this literature review is to shed light on the development of biochemical knowledge in the Lviv region and on prominent figures in the development of biochemistry during the Second World War. PATIENTS AND METHODS: Materials and methods: Review of literature published before 2020. We searched the literature using the search terms 'biochemists', ' Lviv National Medical University', 'second World War'. CONCLUSION: Conclusions: The development of biological research in Lviv can be divided into two historical stages: 1) from the beginning of the founding of Lviv University in 1661 to the First World War; 2) between the First and Second World Wars and after the Second World War. Biochemical research was initiated at the Medical Faculty of Lviv University. In 1939, the Lviv State Medical Institute was established on the basis of the Medical Faculty of the University, where a powerful department of biochemistry functioned, which was headed by a worldclass biochemist - Jakub Parnas.

[Do the old fears return? : An investigation into the experience of the corona crisis among survivors of the Hamburg Firestorm (1943)]. / Kommen die alten Ängste wieder? : Eine Untersuchung zum Erleben der Corona-Krise bei Überlebenden des Hamburger Feuersturms (1943).

BACKGROUND: The generation of war children of the Second World War is currently in old age experiencing the lock-down caused by the coronavirus crisis. OBJECTIVE: How are the restrictions of the lock-down experienced against the background of the war experience? METHODS: A total of 120 witnesses of the Hamburg Firestorm (1943) were asked about their experiences of the corona pandemic by means of a questionnaire in May 2020 and December 2020. Findings from telephone conversations with several witnesses, who regularly participate in a discussion group, have also been taken into consideration for this study. RESULTS: Of the interviewees contacted in May 2020 and December 2020, 98 (82%) and 77 (64%), respectively, sent back the questionnaire, 58 (45) female and 40 (32) male, the mean age was 86.5 years (87.1 years). According to the questionnaire most of them feel relatively stable and confident about their general situation in the pandemic and are mostly concerned with the contact restrictions rather than with their own health. The majority fear negative economic consequences for Germany. About 13% fully agree that the current crisis reminds them of their experiences in the Hamburg Firestorm. As telephone conversations have shown the memories and experiences of the war and the post-war period in general, seem to act as the leading frame of reference for dealing with the current crisis. CONCLUSION: The findings point to typical psychological processing patterns in a war-burdened generation, when they now relate their experiences in the war to the experiences in the corona crisis.

La enfermería durante la Segunda Guerra Mundial. Historia de Vivian Bullwinkel / Nursing during Secord World War. Vivian Bullwinkel´s story

Las enfermeras han tenido un papel muy importante a lo largo de la historia, especialmente, en los conflictos bélicos, siendo claves en la atención y cuidados de los pacientes. Las guerras tienen como consecuencia, entre otras, el desarrollo del cuidado y terapéutica, cuya finalidad siempre ha sido ofrecer la mejor atención para la reincorporación de los soldados al campo de batalla [Fragmento de texto] (AU)

The Guinea Pig Club: Forces and factors that revolutionised burns plastic surgery.

The 'Guinea Pig Club' has been described as the most exclusive club in the world, but the entrance fee is something most men would care not to pay, and the conditions of membership are arduous in the extreme. The Guinea Pig Club was formed in July 1941 to support aircrew who were undergoing reconstructive plastic surgery after receiving burn injuries in the Second World War. This paper will explore the novel surgical techniques employed to treat the Guinea Pig Club members, their experiences at the hospital and their lives after the war and how Sir Archibald McIndoe and the Guinea Pig club members revolutionised burns plastic surgery as we know it today.

Intra-bone nuclear DNA variability and STR typing success in Second World War 12th thoracic vertebrae.

Bones are an important source of DNA for identification in forensic medicine, especially when the remains are skeletonized, which is the case when dealing with victims of the Second World War. Often the amount of bone available for sampling is limited, and therefore it is crucial to sample the bone segment with the highest adequate DNA quantity for identification. Studies performed on all representative skeletal element types of the human body showed that the amount of DNA obtained from different skeletal elements of different body regions varies greatly. When bones from torso were analyzed, thoracic vertebrae outperformed other vertebrae (cervical and lumbar) and, alongside the first ribs, were among the most appropriate bone elements for identification purposes. It was also shown that the quantity of DNA varies significantly within a single bone type. This study focused on exploring intra-bone DNA variability between five parts of 12th thoracic vertebrae (laminae + spinous process, pedicles + transverse processes, and corpus right, left, and middle). The research was based on the theory that the distribution of body weight and consequently bone remodeling, as well as the ratio between cancellous and cortical bone, contribute to different quantities of DNA in different parts of vertebra sampled. The vertebrae were cleaned and cut into five parts, and each part was completely ground to obtain homogenous bone powder. Half a gram of powder from each part was decalcified using a full demineralization extraction method. The DNA was purified in a Biorobot EZ1 machine (Qiagen). DNA quantity and quality were determined using the PowerQuant System (Promega) and autosomal STR typing success using the GlobalFiler Amplification Kit (Applied Biosystems). Thirty-five 12th thoracic vertebrae were sampled from a single Second World War mass grave. The best results with the highest DNA quantity were found in laminae and the spinous process, and among them all vertebrae analyzed yielded full STR profiles except three, where only a few dropouts occurred. The second-ranked bone part was the pedicles and transverse processes. The comparison of DNA degradation in the vertebral segments analyzed does not show statistically significant differences. Considering our research, when only the torso is available for identification, the 12th thoracic vertebra should be collected and the vertebral arch should be sampled for genetic analyses.

History of non-physician anaesthesia providers in Papua New Guinea: from heil tultuls to Anaesthetic Scientific Officers.

The most recent estimates, published in 2016, have indicated that around 70% of anaesthesia providers in Papua New Guinea are non-physician anaesthetic providers and that they administer over 90% of anaesthetics, with a significant number unsupervised by a physician anaesthetist. Papua New Guinea has a physician anaesthetist ratio estimated to be 0.25 per 100,000 population, while Australia and New Zealand have a ratio of 19 physician anaesthetists per 100,000, which is 75 times that of Papua New Guinea. To reach a ratio of seven per 100,000, recommended as the minimum acceptable by the Lancet Commission in 2016, there will need to be over 35 practitioners trained per annum until 2030, at a time when the average annual numbers of recent years are less than three physicians and less than five non-physician anaesthetic providers. We review the development of anaesthesia administered by non-physician indigenous staff and the stages of development from heil tultuls, dokta bois, liklik doktas, native medical assistants, aid post orderlies, and Anaesthetic Technical Officers up to the current Anaesthetic Scientific Officers having attained the Diploma in Anaesthetic Science from the University of Papua New Guinea.

Identification of a Slovenian prewar elite couple killed in the Second World War.

Genetic identification of a Slovenian prewar elite couple killed in 1944 was performed by typing autosomal and Y-chromosomal STRs, and phenotypic HIrisPlex SNPs for hair and eye color prediction were analyzed for the female skeleton using next-generation sequencing (NGS) technology. The clandestine grave containing the couple's skeletal remains was found in 2015 and only the partial remains were found. Living distant relatives could be found only for the male victim. Because of a lack of comparative reference samples, it was not possible to identify the female victim through autosomal and mitochondrial DNA typing. However, the possibility of comparison of eye and hair color with a painting exhibited in the City Museum of Ljubljana by the prominent Slovenian painter Ivana Kobilca existed. Nuclear DNA obtained from the samples was quantified using the PowerQuant System, and then STR typing was carried out with different autosomal and Y-STR kits. From 0.09-9.36 ng DNA/g of powder was obtained from teeth and bones analyzed. Complete autosomal and Y-STR profiles made it possible to identify the male skeleton via comparison with two nephews. For the female victim, predicted eye and hair color was compared to colors on the painting. Kobilca's painting confirms the genetically predicted eye and hair color. After more than seventy years, the skeletal remains of the couple were handed over to their relatives, who buried the victims with dignity in a family grave.